Depth Filters Handle Single-Stage Clarification of Pretreated Feed Streams

EMD Millipore, the life science division of Merck KGaA of Darmstadt, Germany, introduced the Clarisolve depth filter for single-stage clarification of pretreated feed streams. The Clarisolve depth filter is a clarification device with a gradient density structure specifically designed to the particle size distributions of pretreated feed streams.

Clarisolve depth filters process pretreated feeds in a reduced footprint without the need for the secondary stage of clarification. The use of Clarisolve depth filters eliminates the need for centrifugation, which enables implementation of a fully single-use process train and also reduces the pre-use flushing requirements. This technology was developed to address the shortcomings of traditional downstream clarification approaches when processing high cell density and high-product titer cell cultures.

EMD Millipore has also developed a cationic pDADMAC (poly diallyldimenthyl ammonium chloride) flocculation reagent targeted for biopharmaceutical applications which can be used as a pretreatment in combination with the Clarisolve depth filters. pDADMAC treatment, in combination with Clarisolve depth filters, provide a solution for harvesting high cell density cultures and can be incorporated into current clarification platforms.