We currently use a filter chain - #2, 2.5um bag filter, followed by a 1.0um 20 in depth filter and 0.2um 20 in absolute depth filter. We are processing bacterial lysates to separate the flocculent material (protein and large DNA) from the liquid (containing small molecules). With the setup above, the #2 bag filter clogs / cakes after 60-80 L processing. We have to stop processing and change the bag filter before we can continue. The depth filters normally do not clog. We would like to process 200L bacterial lysate without stopping to change the #2 bag filter. Do you have any suggestions for alternatives (different technique or different primary filtration) to improve capacity so that we can filter 200L lysate without needing to stop / change filter units?
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Ernest Mayer Forum Moderator 52 Posts
Re: What filter bags do you suggest to prevent caking and clogging?30 April 2012 at 1:29pmYes. Use high capacity, high area bags. Ones from leading vendors have about 60 ft2 area/bag versus about 3.4 ft2 for a normal size#2 bag.